MATERIALS AND METHODS

The
research will be conducted in two different environments. One is green house
and second is Seed Science Laboratory of department Seed Science and
Technology, in University of Agriculture, Faisalabad under Completely
Randomized Design (CRD). Seeds will be collected from main seed storage of department
of Plant Breeding and Genetics of University of Agriculture, Faisalabad.

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In
laboratory conditions water will be used as a priming agent in this research.
Firstly tube well water from districts of Lahore, Faisalabad, Sheikhupura and
Pattoki will be collected. PH of each sample will be measured with the help of
pH meter. PH meter and beaker in which the pH is going to be measured should
properly be washed with the distilled water to wash off any contaminants
attached to its surface so that the original pH of the sample water remain
undisturbed. The effect of these water samples will be monitored on four
different varieties of wheat seeds separately. The varieties used in this
research will be UJALA, Chakwal, Punjab 2011 and AAS 2017. Before soaking the
seeds in the water 200 seeds per variety will be weighed with the help of
weight balance. Then seeds of every variety will be soaked in district water
samples for 9-10 hours and aeration will be provided with the help of aquarium
pump. The treated seeds will then be dried under shade for 1-2 days. Dried
seeds will then be weighed again using weight balance. From each treatment, 30
seeds were taken and placed on double layer of filter paper thoroughly
moistened, in petri dishes. Petri dishes were properly sterilized before the
use. Petri dishes containing seeds will be provided with the temperature of 21 ±
1o C in the growth chamber for appropriate germination. Seed will be
regarded as germinated after the radicle protrusion of 2mm.

In
the green house sand will be used as a growth medium in this research. Four
wheat varieties will be used to examine the effect of different district water.
The same procedure, as mentioned above, will be followed for the priming of
seeds. After that sand will be sterilized by washing it thoroughly, thrice. A
standard pot of capacity 5-6kg will be utilized for all the treatments. 50
seeds of each variety, treated with all four district water separately, were
sown at uniform depths.

The parameters used
to determine the effect of priming on different wheat seed varieties will
include; germination percentage, shoot length (cm), leaves per plant, leaf area
(cm2), root length, seedling vigor, fresh weight and dry weight.