Species of Limodorum are extremely difficult to grow, as they either germinate with a great amount of difficulty, or they do not germinate at all. This experiment focused on how methods of seed sterilization-scarification and on various sources of nitrogen in seed germination and the development of seedlings. The researchers used different percentages of sodium hypochlorite on the seeds in order to see which had a larger positive effect on the seeds. The compound had been commonly used in the sterilization of orchid seeds, and had been proven to help break the seed dormancy and had a stimulatory effect on the germination of certain orchid species. The researchers collected samples of seeds and proceeded to use sodium hypochlorite to sterilize the seeds.
The percentages of 5 and 1 were used in a solution of sodium hypochlorite and water to test the effect on the seeds. After the seeds had germinated and began to develop, the researchers began to analyze the results. The conclusion was reached that the 1% solution was virtually ineffective on the seeds, and it made no difference at all. The 5% solution; however, caused improvement and showed germination rates that were significantly higher than the normal rate.
It was decided that the increased rate was caused by the large amount of inorganic nitrogen contained with in it versus the 1% solution. This experiment proved the vital need for sterilization if the seeds are to germinate at any rate, especially a high rate. It also proved the benefit of a large amount of nitrogen being used on seeds as a pre-treatment. If this experiment were to be conducted again, it is believed that included a broader range of percentages could have been beneficial to the experiment. This is believed because there could potentially be another percentage that increases the rate of germination by an even greater amount.
Overall, the experiment was well conducted and did not need many modifications.